Serum response factor regulates smooth muscle contractility via myotonic dystrophy protein kinases and L-type calcium channels
Fig 3
Altered expression of Celf1 transcriptional variants in Srf KO SMC.
(A) A genomic map of Celf1 variants expressed in jejunum SMC. Exons are numbered 1–19. Four exons containing alternative transcription start sites are shown by a star (*). Two variable regions (V1 and V2) with the predominant transcriptional start sites are indicated. (B) Expression levels of Celf1 variants in SMC and jejunal and colonic tissue, obtained from RNA-seq data [6]. (C) qPCR data showing expression changes of Celf1 V1 and V2 transcripts at KO days 5, 10, and 15 (n = 3). (D) Comparison of expression levels of Celf1 and Mbnl1 in WT jejunum, colon, and their isolated SMC, obtained from RNA-seq data [6]. (E) Induction of CELF1 protein in KO muscle. Amount of protein expression was obtained from LS-MS/MS proteomics data.