A novel flow cytometry-based assay for the quantification of antibody-dependent pneumococcal agglutination
Fig 1
Agglutination of S. pneumoniae by anti-capsule antibodies can be detected by flow cytometry.
S. pneumoniae TIGR4 were incubated with a serotype specific polyclonal rabbit antiserum (serotype 4; α-ST4; right panel) and with rabbit antiserum against a heterologous serotype (serotype 14; α-ST14; left panel). Agglutination was assessed by flow cytometry and is represented as dot plots of the FSC-A vs the SSC-A (A) and histograms of the FSC-A signal (B) and by phase contrast microscopy (C).