DNA Polymerases ImuC and DinB Are Involved in DNA Alkylation Damage Tolerance in Pseudomonas aeruginosa and Pseudomonas putida
Fig 4
Sensitivity of P. putida ΔalkA and ΔalkAtag strains and their different TLS polymerase-deficient derivatives to MMS and MNNG.
Sensitivity was estimated by spotting 10-fold dilutions of overnight cultures of P. putida ΔalkA (A, B) and ΔalkAtag (C, D) strains onto LB plates containing different concentrations MMS (A, C) and MNNG (B, D) and incubated at 30°C for 24 h. Data represents the mean (±95%CI) values. (●) ΔalkA; (■) ΔimuCalkA; (▲) ΔdinBalkA; (▼) ΔimuCdinBalkA (A, B); (●) ΔalkAtag; (■) ΔimuCalkAtag; (▲) ΔdinBalkAtag; (▼) ΔimuCdinBalkAtag (C, D). Asterisks indicate statistically significant difference (P < 0.05; two-way ANOVA followed by Tukey’s multiple comparison post hoc test) in the sensitivity of the ΔalkA or the ΔalkAtag mutant in comparison with the other deletion mutants. In addition, significant difference in sensitivity between the ΔdinBalkA or ΔimuCalkA and the ΔimuCdinBalkA (A); ΔdinBalkAtag or ΔimuCalkAtag and the ΔimuCdinBalkAtag strains (D) is indicated.