Creation of Novel Protein Variants with CRISPR/Cas9-Mediated Mutagenesis: Turning a Screening By-Product into a Discovery Tool
Fig 2
Validation of gain-of-function MAP2K1 alleles.
A375 cells constitutively expressing lentiviral-delivered ORFs were assessed for ATP levels after 96 hours of drug treatment, normalized to no drug. Negative controls are shown in gray, positive controls in green, and test ORFs in blue. For vemurafenib, the doses used were, left to right, 0, 0.5, 1, 2, 6, 8, and 10 μM. For selumetinib, the doses used were, left to right, 0, 0.05, 0.1, 0.200, 0.600, 1, and 2 μM.