Derivative of Extremophilic 50S Ribosomal Protein L35Ae as an Alternative Protein Scaffold
Fig 1
Amino acid sequences for the recombinant forms of L35Ae from P. horikoshii used in this study.
Secondary structure elements of L35Ae from P. furiosus are indicated (refer to PDB entry 2lp6 [34]): β-sheets 1–6, CDR-like loops 1–3, α-helix (green). The residues affected by randomization are shown in color. (A) The amino acid sequences of rWT L35Ae and its 10X mutant [30]. The residues differing between 10X and rWT L35Ae are indicated using bold font. Two 10X variants were used in the phage display library, which contain a loop 2 of original length (a) or elongated by three residues (b). (B) The amino acid sequences of L35Ae 10X with C-terminal GLE sequence replaced by myc tag (‘10X-myc’) and those for HEL-specific binders L4 and L7, isolated from the phage display library of L35Ae 10X. The regions subjected to randomization are indicated in pink.