Anti-Prion Screening for Acridine, Dextran, and Tannic Acid using Real Time–Quaking Induced Conversion: A Comparison with PrPSc-Infected Cell Screening
Fig 1
Optimization of RT-QuIC and determination of compound cytotoxicity.
Time point optimization was performed using CSF samples from 24 cases of progressive dementia (A) and validation was performed using serially diluted sCJD brain homogenate (B) and CSF from a sCJD or AD patient (C). Cell viability was assessed in the presence of 5 different compounds at 5 different concentrations between 1 μM and 100 μM (D). Values represent the mean of 3 independent experiments and the standard deviation is shown as error bars, P < 0.05.