Lysine Decarboxylase with an Enhanced Affinity for Pyridoxal 5-Phosphate by Disulfide Bond-Mediated Spatial Reconstitution
Fig 4
Comparison of resistance to temperature between SrLDCWT and SrLDCA225C/T302C.
(A) Thermal stability measurement of SrLDCWT and SrLDCA225C/T302C. The Tm values of SrLDCWT and the SrLDCA225C/T302C mutant are displayed. (B) (C) Comparison of thermal stability between SrLDCWT and SrLDCA225C/T302C. The LDC activities of SrLDCWT and the SrLDCA225C/T302C mutant are measured using proteins pre-incubated at 37°C (B) and 60°C (C).