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Lysine Decarboxylase with an Enhanced Affinity for Pyridoxal 5-Phosphate by Disulfide Bond-Mediated Spatial Reconstitution

Fig 4

Comparison of resistance to temperature between SrLDC^WT and SrLDC^A225C/T302C.

(A) Thermal stability measurement of SrLDC^WT and SrLDC^A225C/T302C. The T_m values of SrLDC^WT and the SrLDC^A225C/T302C mutant are displayed. (B) (C) Comparison of thermal stability between SrLDC^WT and SrLDC^A225C/T302C. The LDC activities of SrLDC^WT and the SrLDC^A225C/T302C mutant are measured using proteins pre-incubated at 37°C (B) and 60°C (C).

doi: https://doi.org/10.1371/journal.pone.0170163.g004