A Split-GFP Gateway Cloning System for Topology Analyses of Membrane Proteins in Plants
Fig 3
The plasma membrane syntaxin, PEN1, has its N-terminus in the cytosol and C-terminal in the apoplast.
(A-F) Constructs encoding GFP1-10, SP-GFP1-10-HDEL, SP-GFP1-10, GFP11-PEN1 and PEN1-GFP11 were co-transformed pairwise into cells of Nicotiana benthamiana leaves by agroinfiltration. Confocal microscopy was performed 2 days after infiltration. Scale bars, 20 μm. Arrow, nucleus.