The Green Tea Catechin Epigallocatechin Gallate Ameliorates Graft-versus-Host Disease
Fig 4
Impact of EGCG on systemic inflammation during GVHD.
1,5x107 BM and 2x106 T cells from LP/J mice were injected into C57BL/6 mice after busulfan and cyclophosphamide conditioning. (a) Sections of colon and liver at day +17 were stained with CD4 or CD8 antibody, and at least 6 representative pictures per animal were evaluated. CD4 positive cells were increased and CD8 positive cells were decreased as a result of EGCG treatment. (b) Representative staining of colon with anti-CD4 antibody. Abundant CD4 positive cell infiltration in colon sections of mice treated with EGCG. Sections were counterstained with 4`, 6-Diamino-2-phenylindole. (c) Regulatory T cells during GVHD. FACS analysis of blood and lymph nodes and section analysis of colon of mice treated or non-treated with EGCG. Tregs were increased in blood and colon of EGCG treated mice. (d) Leukocytes during GVHD. FACS analysis of blood and spleens of myeloid and lymphoid cells during GVHD. Within analyzed cells (B cells, monocytes, granulocytes and NK cells), monocytes were significantly decreased in the blood of EGCG treated mice. Shown is data from one representative experiment, n = 8 (ns = not significant, *p <0.05, **p<0.01 ***p<0.001).