Zinc Modulates Endotoxin-Induced Human Macrophage Inflammation through ZIP8 Induction and C/EBPβ Inhibition
Fig 5
Zinc inhibits LPS-induced C/EBPβ.
During LPS challenge zinc alters (A) C/EBPβ nuclear accumulation and phosphorylation beginning at 5 min and out to at least 30 min as determined by Western analysis of nuclear extracts and reduces both (B) C/EBPβ activity at 5 min as determined by TransAM ELISA of nuclear extracts and (C) transcription of C/EBPβ at 6h as determined by qRT-PCR. Zinc reduces (D) ERK phosphorylation at 60 min, but does not significantly alter (E) p38 phosphorylation (F) or p105/p50 degradation, as determined by Western analysis of whole cell lysates, following 5, 15, 30 or sixty min co-stimulation with LPS (100 ng/mL) and ZnSO4 (18 μM). Panels A, D, E and F are paired with densitometry and representative of 3 experiments. Panels B and C are cumulative data from 3 donors (mean ± SEM; *p < 0.05).