Splenic CD4+ T Cells in Progressive Visceral Leishmaniasis Show a Mixed Effector-Regulatory Phenotype and Impair Macrophage Effector Function through Inhibitory Receptor Expression
Fig 8
Ex vivo blockade of PD-L2 reduces parasite burden.
Spleen cells from 28-day chronically infected hamsters were cultured with αPD-L2 antibody or isotype control for 48 hours. The parasite burden (18s mRNA expression) and expression of iNOS, IFNγ, Arg1 and IL-10 were determined by real time RT-PCR. Results are expressed as a relative fold-increase of the αPD-L2 antibody or isotype control groups compared to infected splenocytes at 0 hours. Shown is the mean and SEM of two independent experiments with 6 replicates per group.