High Concentrations of Angiopoietin-Like Protein 4 Detected in Serum from Patients with Rheumatoid Arthritis Can Be Explained by Non-Specific Antibody Reactivity
Fig 1
Analysis of the distribution of ANGPTL4 immunoreactivity after separation of human serum by SEC.
Serum from one patient with rheumatoid arthritis (RA) and a high level of ANGPTL4 reactivity (A) or from a healthy age and sex matched donor (B) was subjected to chromatography on a Superose® 6HR column. ANGPTL4 immunoreactivity in the fractions was measured by the ELISA using antibody XPQ0109091 (lot I) for detection (solid lines). Concentrations of total cholesterol (TC, dashed lines) and triacylglycerol (TG, dotted-lines) were measured in the fractions to localize the elution positions of VLDL, LDL and HDL (indicated by arrows). The highest levels of ANGPTL4 reactivity were found in fractions obtained from RA-serum and eluted in a position between that of VLDL and LDL.