The CCL2/CCR2 Axis Affects Transmigration and Proliferation but Not Resistance to Chemotherapy of Acute Myeloid Leukemia Cells
Fig 3
THP-1 cell migration in response to CCL2.
(A) THP-1 cells were seeded on the upper chamber of a transwell plate and incubated with various concentrations of CCL2 (1, 5, 10, 50, 100 and 200 ng/ml) in the lower chamber overnight to measure THP-1 cell migration. (B) THP-1 (CCR2+) and U-937 (CCR2-) cell migration study was performed by exposing leukemia cells to CCL2 (10 ng/ml), sc-202525 (5 nM) (CCR2 inhibitor) or SDF-1 (200 ng/ml) (CXCR4 inhibitor, positive control) overnight. (C) THP-1 cells were exposed to CCL2 (10 ng/ml), anti-CCL2 (5 μg/ml), anti-CCR2 (10 μg/ml), sc-202525 (5 nM) and isotype control antibody overnight. Leukemia cell migration for each study was measured by FCM. Each bar represents the mean ± SD of 3 independent experiments (***p < 0.001).