Anti-Inflammatory Effects of Rosiglitazone in Obesity-Impaired Wound Healing Depend on Adipocyte Differentiation
Fig 3
Cytokine-induced CXCL2 is dependent on adipocyte differentiation.
Cultured 3T3-L1 pre-adipocytes were differentiated in DMI and DMII for 12 days. Final differentiation was assessed by Red Oil staining (a). Non-differentiating and differentiating 3T3-L1 adipocytes were assessed by qRT-PCR analysis for FABP4 mRNA expression (b) and leptin protein release into culture media (c) at the indicated time points. **, p < 0.01; (Student’s unpaired t test) compared to non-differentiating cells. Bars indicate the mean ± S.D. obtained from four independent experiments (n = 4). Non-differentiating and differentiating 3T3-L1 adipocytes were stimulated with fresh medium in the absence or presence of cytokines (25 ng/ml IL-1β, 50 ng/ml TNFα) for 8h at the indicated time points. Conditioned media from the cells were analyzed by ELISA for CXCL2 (d) and CCL5 (e) protein expression. **, p < 0.01; n.s., not significant (Student’s unpaired t test) compared to cytokine-treated non-differentiating cells. Bars indicate the mean ± S.D. obtained from four independent experiments (n = 4).