Do Exogenous DNA Double-Strand Breaks Change Incomplete Synapsis and Chiasma Localization in the Grasshopper Stethophyma grossum?
Fig 2
Double immunolocalization of γ-H2AX (green) and RAD51 (red) in squashed spermatocytes of S. grossum irradiated with 5Gy dose of gamma rays.
The chromatin is counterstained with DAPI (blue). (A-D) Late leptotene. Immunolocalization of γ-H2AX and RAD51 one hour after irradiation. Whereas γ-H2AX labelling is distributed in the whole chromatin, RAD51 foci are concentrated in a nuclear region. (E-H) Zygotene. One day after treatment. The labelling of γ-H2AX covers the whole nucleus but that of RAD51 is polarized in a nuclear area. (I-L) Late zygotene. Three days after treatment. The distribution of γ-H2AX and RAD51 is maintained. All images are the sum of all focal planes of a nucleus. Bar represents 10 μm.