An Anti-Human Lutheran Glycoprotein Phage Antibody Inhibits Cell Migration on Laminin-511: Epitope Mapping of the Antibody
Fig 5
Effects of anti-Lu phage antibodies on tumor cell migration.
(A) Flow cytometric analyses using anti-Lu phage antibodies. The transfectants expressing Lu and HuH-7 cells were incubated with the indicated primary antibodies (A7, 2.0 × 1010 pfu/2.0 × 105 cells; C7, 2.0 × 1010 pfu/2.0 × 105 cells; BRIC221, 10μg/2.0 × 105 cells) and then with Alexa 488-conjugated secondary antibody for flow cytometric analysis. The expression of Lu is shown as a solid line. The gray area indicates the negative control. (B) Migration of A549 cells on LM-511 in the presence of either mAb87207 or the anti-Lu phage antibodies. After the cells adhered to substrata coated with LM-511 (0.8 nM), antibodies were added to the media (A7, 1.2 × 1011 pfu/ml; C7, 5 × 1010 pfu/ml; mAb87207, 10 μg/ml). Cell movements were monitored by time-lapse video microscopy and were quantified as described in Materials and Methods. Ten cells were randomly selected in each field. *, P < 0.01 by t test.