An Anti-Human Lutheran Glycoprotein Phage Antibody Inhibits Cell Migration on Laminin-511: Epitope Mapping of the Antibody
Fig 4
Characterization of anti-Lu phage clones.
(A) ELISA using anti-Lu phage clones and mAb87207. Each anti-Lu phage clone was mixed with the diluted antibody at 0.5, 1.0, and 2.0 μg/ml and was added to the wells coated with Sol-Lu. Except for A7, all the phage clones could compete with mAb87207, suggesting that they could inhibit the binding of Lu to laminin α5. (B) Inhibitory effects of the anti-Lu phage clones, A7 and C7, on the binding of Lu-Fc to laminin α5. Binding assays on tissue sections were performed, and the Lu-Fc bound to laminin α5 was quantified as described in Materials and Methods. The binding of Lu-Fc (1 μg/ml) was set to 1.0 in the absence of the anti-Lu phage clones. Each bar represents the mean of triplicate assays. Error bars indicate standard deviation. *, P < 0.05 by t test.