SLC7A5 Functions as a Downstream Target Modulated by CRKL in Metastasis Process of Gastric Cancer SGC-7901 Cells
Fig 4
Depletion of CRKL in SLC7A5 cells transfected by pGU6/Neo/siCRKL vector.
(A) Immunochemistry staining of cells was carried out to exam the stable transfected SGC-7901 cells (400×). The staining intensity of SGC-7901 cells transfected with CRKL-shRNA (SGC-7901/shCRKL) were significantly decreased compared with the negative control cells. (B) The QRT-PCR assay results indicated that CRKL mRNA level was significantly decreased compared with the negative control (**P<0.01). (C) The Western blot analysis results presented a significant suppression of CRKL expression after transfection (*P<0.05).