Blocking Nerve Growth Factor Signaling Reduces the Neural Invasion Potential of Pancreatic Cancer Cells
Fig 6
Reciprocal signaling between pancreatic cancer cells and neurites extended from the PC-12 cells is reduced when the NGF-TRKA signaling pathway is disrupted.
A) Treatment with the NGF neutralizing antibody or the TRKA inhibitor (GW441756) reduces the neurite outgrowth induced by conditional medium from pancreatic cancer cells. B) Quantification of neurite outgrowth assay shown in A. Conditioned Media (CM) collected from MiaPaCa2 or BxPC3 cells were treated with a neutralizing NGF antibody and a TRKA inhibitor and neurite (yellow arrows) extension from the PC-12 cells was visualized under a bright field microscope. Recombinant NGF protein was used as a positive control. *≤ 0.01; ** ≤ 0.001; *** ≤ 0.0001 (compared to Conditioned Media).