Derivation of Corneal Keratocyte-Like Cells from Human Induced Pluripotent Stem Cells
Fig 6
Limbal rim seeded NCCs adopt a corneal keratocyte-like fate and migrate between the collagen lamellae.
(A) Control non-seeded rim cryosections showed no DAPI+ nuclei or AP2a+/ Keratocan+ cells. Limbal rims seeded with derived NCCs did contain AP2a+/ Keratocan+ cells after 21 days of culture (arrows). (B) High magnification images show Keratocan+ derived NCCs migrated within the collagen lamellae of the stromal layer of the cornea (this panel is a higher magnification of the Keratocan staining shown in (A). ABCB5+, Vimentin+ and HNK1+ staining was also detected in these cells. Scale bars in (A) represent 100 μm, scale bars in (B) represent 50 μm.