Forms and Amounts of Vitamin B12 in Infant Formula: A Pilot Study
Fig 2
The forms of B12 in infant formula no. 3 (NAN1).
B12 was extracted in the dark and subjected to HPLC. B12 in each post-column fraction was measured by an in-house ELISA. The positions of elution for hydroxo-B12 (HO), cyano-B12 (CN), 5’-deoxyadenosyl-B12 (Ado), and methyl-B12 (CH3) are indicated. B12 eluted predominantly as hydroxo-B12 and cyano-B12 in all infant formulas tested. The graphics were created in Graph Pad Prism version 5.