Lowered Expression of Tumor Suppressor Candidate MYO1C Stimulates Cell Proliferation, Suppresses Cell Adhesion and Activates AKT
Fig 4
siRNA-knockdown of MYO1C enhanced viability.
(a) Immunoblot of MYO1C protein expression in MCF10A cells transfected with MYO1C-siRNA and negative control (scrambled siRNA) to the final concentration of 10nM at 24, 48, 72 and 96 hours post-transfection. The image is a representative from at least three independent experiments. (b) Histogram showing percentages of relative cell viability of MCF10A cells transfected with MYO1C-siRNA and scrambled siRNA to the final concentration of 10nM. Significant increase in the number of live cells was observed at 48, 72 and 96 hours post-transfection compared to cells transfected with the scrambled siRNA. Each bar represents mean value ± SEM of all repeats (at least three independent experiments, each in quadruplicates). A Student’s t-test was used to compare the differences in mean value, ****P-value < 0.001 versus cells treated with scrambled siRNA as well as untreated cells (time point zero).