Extracellular Redox Regulation of Intracellular Reactive Oxygen Generation, Mitochondrial Function and Lipid Turnover in Cultured Human Adipocytes
Fig 7
Shifting redox state towards oxidative conditions early in adipogenesis in human adipocytes increased final lipid content.
Human preadipocytes were differentiated as detailed previously [17]. The media were supplemented with 55 μM GSSG. The induction media and the GSSG were added either on day 0, 5, 9 or 12 of differentiation. All cells were differentiated for a total of 14 days and fat accumulation was assessed on day 14 by Oil Red O staining by extracting the dye and quantifing as described [24]. Data were pooled from 2 experiments done with 8 replicates and expressed as % of control calculated as mean ± SD. The addition of GSSG at the start of differentiation significantly (p = 0.003) increased fat accumulation by day 14. On the other hand, addition of GSSG at day 12 significantly (p = 0.02) reduced lipid accumulation. ANOVA analysis with a post-hoc Dunnetts test was used for statistics.