Depletion of Neutrophils Promotes the Resolution of Pulmonary Inflammation and Fibrosis in Mice Infected with Paracoccidioides brasiliensis
Fig 6
Depletion of neutrophils is associated wit a reduction on the fungal burden in lungs, liver, and spleen from mice infected with P. brasiliensis at the chronic stages of infection.
Fungal load measurement was performed in lung, liver and spleen of mice at 12 weeks post-challenge with 1.5x106 P. brasiliensis (Pb18) yeast cells and treated with an isotype control Ab or anti-Ly6G mAb at 4 weeks (A, B, C) or 8 weeks (D, E, F) post-challenge. Data shown represent median and IQR (n = 4–5 mice/group; representative of two independent experiments). A statistically significant reduction in fungal burden was observed in the lungs of mice infected and treated with the anti-Ly6G mAb (*, P<0.05) compared to the infected untreated mice and the infected-isotype control Ab treated mice. Pb18, infected, untreated mice; Pb18 + Iso, infected mice treated with isotype control Ab (clone: 2A3); Pb18 + anti-neutrophils, infected mice treated with anti-Ly6G mAb (clone: 1A8).