Complex Interplay between FleQ, Cyclic Diguanylate and Multiple σ Factors Coordinately Regulates Flagellar Motility and Biofilm Development in Pseudomonas putida
Fig 8
Gel mobility shift assays of FleQ on the PlapA and PbcsD promoters.
Top: Cartoon of the probes used for each of the promoter regions, indicating the restriction enzymes used for cleavage, the sizes (in bp) of the resulting fragments, and the location of the predicted FleQ binding motifs (open boxes). Drawn to scale. Bottom: Ethidium bromide-stained PAGE showing the results of a typical gel mobility shift assay. Each promoter region was challenged with 0, 0.45 and 4.5 μM FleQ.