Activation of Ftz-F1-Responsive Genes through Ftz/Ftz-F1 Dependent Enhancers
Fig 5
Identification of candidate Ftz/Ftz-F1 dependent enhancers.
A screen shot from Flybase GBrowse showing genomic regions around Ftz-F1-responsive genes. Published BDTNP data available at http://bdtnp.lbl.gov/Fly-Net/browseChipper.jsp was downloaded and all interval coordinates along with the peak binding positions were remapped from release 4 to release 6 of the Drosophila genome using the Coordinate Converter provided by Flybase. The Ftz binding data was then uploaded into Flybase GBrowse for visualization. Candidate enhancers (red arrows) were identified using three major criteria: 1) Location near Ftz-F1-responsive target gene (light blue); 2) Ftz ChIP-chip binding, yellow arrow (1% FDR) or orange arrow (25% FDR); 3) candidate Ftz-F1 binding sites (thin purple arrows). Open chromatin (green arrows), was also found at blastoderm stage at all enhancers. Enhancer chromosomal locations (flybase v. 6) are: (A) ken 2R:23868513..23869466, (B) en 2R:11524814..11525958, (C) aay 3L:9416225..9417230, (D) mid 2L:5473726..5474732, (E) 5-HT2A 3R:4612279..4614294, (F) trn 3L:13073977..13075024, (G) hh 3R:23138154..23139164, (H) Antp 3R:6948758..6949766, (I) blot 3L:17409206..17410251.