Loss of Mll3 Catalytic Function Promotes Aberrant Myelopoiesis
Fig 6
Effects of MLL3 disruption on myeloid cell adhesion and migration.
A, Relative MLL3 expression in human KBM7 cells as determined by qPCR. TaqMan probes (indicated in red) recognized exon junctions before (exons 18–19) and after (exons 39–40 and 55–56) the gene-trap (exon 38), indicated in green. B, Relative adhesion of KBM7 cells to fibronectin as measured in fluorescence units. Shown is the average of technical replicates from four individual experiments + SEM. C, The number of KBM7 cells that migrated across a trans-well membrane in response to FBS after 24h. Data are the combination of technical replicates from four individual experiments + SD. Ref, control gene-trap; MLL3, MLL3-targeted gene-trap. *p<0.05, **p<0.01 as determined by the Mann-Whitney test.