Tanshinone IIA Modulates Low Density Lipoprotein Uptake via Down-Regulation of PCSK9 Gene Expression in HepG2 Cells
Fig 3
Effect of tanshinone IIA on the cell-surface LDLR levels and LDL uptake in HepG2 cells.
HepG2 cells were cultured in LPDS medium and treated with the vehicle (0.1% DMSO) or tanshinone IIA (5 and 10 μM) for 24 h. (A) The level of cell-surface LDLR was determined by flow cytometry analysis. A representative histogram is shown. (B) Summary of cell-surface LDLR levels. (C) BODIPY®-FL-LDL uptake was determined by flow cytometry analysis. A representative histogram is shown. (D) Summary of LDL uptake. The data represent the mean ± SD from three independent experiments. ** p<0.01 represent significant differences compared to the vehicle group.