Analysis of Polymorphic Membrane Protein Expression in Cultured Cells Identifies PmpA and PmpH of Chlamydia psittaci as Candidate Factors in Pathogenesis and Immunity to Infection
Fig 9
PmpA, B, D and H production differs under normal C. psittaci and during penicillin-induced stress culture conditions.
C. psittaci infected HeLa cells were fixed at 12, 18, 24, 32 and 48 hpi, and double-stained with a chlamydial LPS-specific antibody and Pmp-specific antibody. For each Pmp subtype, the percentage of positive inclusions was determined by a macro based on co-localization of the two antigens. The results shown here did not take into account smaller inclusions that are formed at 24, 32 and 48 hpi. The data are expressed as box plots: the box represents the 25th-75th percentiles, the median is depicted by a bar across the box and the whiskers on each box represent minimum and maximum value. Outliers are depicted by dots. Statistically significant differences (P < 0.05) are indicated with an asterix.