Vinpocetine Attenuates the Osteoblastic Differentiation of Vascular Smooth Muscle Cells
Fig 6
PD98059 and LY294002 attenuated the effect of vinpocetine on osteoblastic differentiation of β-GP-stimulated VSMCs.
PD98059 and LY294002 were respectively added into the culture of β-GP-stimulated VSMCs with or without vinpocetine treatment. After a culture of 48h, the cells were harvested for mRNA expression of Runx2 and BMP-2 as well as the ALP activity. Our data showed that both PD98059 and LY294002 significantly inhibited the mRNA expression of Runx2 (A) and BMP-2 (B) as well as the ALP activity (C). After a culture of 18 days, the results of Alizad S staining showed that both PD98059 and LY294002 significantly blockaded the inhibitory effect of vinpocetine on formation of mineralized nodule in calcifying VSMCs. Representative results from three independent experiments were presented.