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The Potential Roles of the G1LEA and G3LEA Proteins in Early Embryo Development and in Response to Low Temperature and High Salinity in Artemia sinica

Fig 8

Western blotting analysis of As-G1LEA, As-G3LEA and Trehalase at different developmental stages.

(A) Western blotting analysis of the expression of As-G1LEA, As-G3LEA and Trehalase proteins at different developmental stages in A. sinica. The intensities of the As-G1LEA, As-G3LEA and Trehalase protein bands were normalized against those of GAPDH. (B–D) Values are expressed as arbitrary units of relative value. The expressions of As-G1LEA, As-G3LEA and Trehalase proteins at 0 h were used as the control. Significant differences for the different development stages (P< 0.05) were analyzed by ANOVA and are indicated by lowercase letters (a, b and c).

Fig 8

doi: https://doi.org/10.1371/journal.pone.0162272.g008