A Model to Explain How the Bacille Calmette Guérin (BCG) Vaccine Drives Interleukin-12 Production in Neonates
Fig 1
Bacille Calmette Guérin (BCG) does not induce IL-12 p35 mRNA production from human neonatal monocyte-derived dendritic cells (mdDCs), but induces IL-12 p40 mRNA production in a Toll-like receptor (TLR)2-dependent manner.
Human umbilical cord blood CD14+ monocytes were differentiated into mdDCs using rIL-4 and rGM-CSF, stimulated with BCG x 18–24 hours, and then cellular mRNA was isolated for qRT-PCR. (a) relative expression of IL-12 p35 mRNA levels upon BCG stimulation compared to unstimulated control. Bars are median values, ‡ p = 0.3, n = 6 independent experiments; (b) relative expression of IL-12 p40 mRNA levels upon BCG stimulation compared to unstimulated control, in the presence or absence of a TLR2 blocking antibody. Bars are median values, * p<0.05 compared to BCG stimulation with isotype control antibody pre-incubation, n = 6 independent experiments.