Hyperglycemia Determines Increased Specific MicroRNAs Levels in Sera and HDL of Acute Coronary Syndrome Patients and Stimulates MicroRNAs Production in Human Macrophages
Fig 4
Expression of Drosha, DGCR8 and Dicer mRNA (A) and of selected miRNAs (B) in human macrophages incubated with normoglycemic CAD patients’ sera with stable angina (SA) or acute coronary syndrome (ACS), with/without added glucose. The expression level of each gene of interest was determined relative to β-actin, while the expression level of each miRNA was determined relative to snRNU6. Data are expressed as mean 2-ΔΔCq values ± standard error of the mean. +Gluc = 5.5 mM (100 mg/dl) added glucose; LPDS = human lipoprotein-deficient serum (*p<0.05, **p<0.01 +Gluc vs. normoglycemic (NG), Student T-test).