Live-Cell, Label-Free Identification of GABAergic and Non-GABAergic Neurons in Primary Cortical Cultures Using Micropatterned Surface
Fig 3
Development of non-GABAergic and GABAergic cortical neurons on micropatterns.
Phase-contrast (A,C) and immunofluorescent (B,D) images of rat cortical neurons grown on Pattern #3. Phase-contrast images were taken every day from 2 to 7 DIV, and were fixed and stained at 7 DIV to retrospectively identify the cell type of each neuron (green, MAP2; red, GAD67). Neurons shown in (A) and (B) were non-GABAergic (GAD67−), and those shown in (C) and (D) were GABAergic (GAD67+). By growing neurons on the micropattern, axon is formed in the longest pathway, i.e., in the upward direction. Scale bar, 20 μm.