Phylogenetic Analysis Supports Horizontal Transmission as a Driving Force of the Spread of Avian Bornaviruses
Fig 2
ABBV-1 sequences from wild aquatic birds form continent-associated clusters.
Partial sequences of N (A, 320 bp) or M (B, 200 bp) genes of ABBV-1 were analysed. Phylogenetic analysis was performed using Neighbor-Joining algorithm and Jukes-Cantor distance model in Geneious R8. The trees were rooted with Bornaviridae references sequences (not shown; see Fig 1). Values at branches represent support in 1,000 bootstrap replicates. Only bootstrap values ≥70 at major branches are shown. Nodes with bootstrap support of ≥90 in additionally performed ML analysis are indicated by asterisks. Sequences depicted in bold were generated during this study. Clusters 3 and 4 were confirmed to be independent by alignment of short overlapping partial M gene sequences (113 bp) from both clusters (data not shown).