Involvement of the FoxO1/MuRF1/Atrogin-1 Signaling Pathway in the Oxidative Stress-Induced Atrophy of Cultured Chronic Obstructive Pulmonary Disease Myotubes
Fig 3
Expression levels of protein synthesis markers after H2O2 treatment.
(A) Variation of IGF-1 mRNA expression in COPD myotubes after H2O2 treatment. (B) Representative Western blots showing expression levels of phosphorylated-AKT (P-AKT) and AKT in cultured COPD myotubes derived from 12 COPD patients (1–12), with or without H2O2 treatment. Tubulin expression is used as a loading control. (C) Variation of the P-AKT/AKT ratio relative to tubulin expression levels following H2O2 treatment. (n.s.) indicates statistically non-significant. The medians are indicated.