Pdcd4 Is Involved in the Formation of Stress Granule in Response to Oxidized Low-Density Lipoprotein or High-Fat Diet
Fig 4
Ectopic Pdcd4 is co-localized with specific markers of SGs in ox-LDL-treated HeLa cells.
HeLa cells were transfected with pEGFP-C1-Pdcd4 in the presence or absence of ox-LDL (50 μg/ml) for 24 h. Intracellular localization of exogenous Pdcd4 was visualized with GFP (green). SG markers (TIA-1, FXR1 and eIF4A) were examined by rhodamine (red) using immunofluorescence. Cells were counterstained with DAPI (blue). The original magnification is 1000. Scale bar = 20 μm. Representative (A) and statistical (B) data are shown. Data represent more than three independent experiments with similar results. ***P <0.001.