Utilisation of ISA Reverse Genetics and Large-Scale Random Codon Re-Encoding to Produce Attenuated Strains of Tick-Borne Encephalitis Virus within Days
Fig 2
Proportion of mice with virus detected in the serum (A) or in the brain (B) and viral RNA yields in sera (C) and in the brain (D) by qRT-PCR. For each group, four sera were sampled at 2 days pi. Brains were samples at 7 and 43 days pi (D7 and D43 in panels B and D; the number of mice sampled for each time point is indicated onto each bar in panel B). Viral RNA yields are expressed as log10 number of copies per mL (sera) or log10 relative number of copies per mL (brains) (values were normalized using the housekeeping gene HMBS as detailed in the Materials and Methods section). For viral RNA yields, white squares represent minimal and maximal values. Black lines and error bars represent mean values +/- standard deviation.