Proteomic and Transcriptomic Analyses of Swine Pathogen Erysipelothrix rhusiopathiae Reveal Virulence Repertoire
Fig 6
Pyruvate detection with LDH assay.
Bacteria were cultured to mid-logarithmic phase in BHI medium and triplicate samples were collected at 3 time points for the virulent strain HX130709 (5 h, 6 h, 7 h) and the attenuated strain HX13079a (8 h, 9 h, 10 h). Lactate dehydrogenase levels and bacterial counts (colony forming units; CFU) were measured as described in materials and methods. Results are expressed as LDH/cfu. Pyruvate levels are assumed to correlate positively with LDH activity.