An Optimized Lock Solution Containing Micafungin, Ethanol and Doxycycline Inhibits Candida albicans and Mixed C. albicans – Staphyloccoccus aureus Biofilms
Fig 7
Polymicrobial regrowth after treatment with OS 1.
Regrowth of mono- and polymicrobial biofilms on silicone disks after treatment with optimized lock solution. Monomicrobial (CA, SA) and polymicrobial (CA+SA) biofilms of C. albicans and S. aureus were challenged with drug free (DF, closed circles) or optimized solution (OS 1, 20% ethanol, 800 μg/mL doxycycline, and 0.015625 μg/mL micafungin, open circles) prepared in sterile growth medium for 24 hours. Immediately following lock solution treatment, disks were briefly sonicated and cultured in selective liquid medium for 24 hours. Serial dilutions were plated onto selective medium to quantify microbial regrowth. Horizontal line in dataset represents the median. Data is cumulative of four independent experiments performed in triplicate. *, P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001 using a Kruskal-Wallis one-way ANOVA and Dunn’s multiple comparisons post-test.