An Optimized Lock Solution Containing Micafungin, Ethanol and Doxycycline Inhibits Candida albicans and Mixed C. albicans – Staphyloccoccus aureus Biofilms
Fig 6
Treatment of mature C. albicans and S. aureus polymicrobial biofilms on silicone disks with OS 1.
Monomicrobial (CA, SA) and polymicrobial (CA+SA) biofilms of C. albicans and S. aureus were challenged with drug free (DF) or optimized solution (OS 1, 20% ethanol, 800 μg/mL doxycycline, and 0.015625 μg/mL micafungin) prepared in sterile growth medium for 24 hours. Metabolic activity was assessed using the XTT assay and reported as % inhibition of the drug-free control. Error bars represent SDs. Data is cumulative of 4 independent experiments performed in triplicate. **, P ≤ 0.01, n.s., not significant using a one-way ANOVA and Tukey’s multiple comparisons post-test.