An Optimized Lock Solution Containing Micafungin, Ethanol and Doxycycline Inhibits Candida albicans and Mixed C. albicans – Staphyloccoccus aureus Biofilms
Fig 5
Prevention and treatment of clinical isolate and reference strain biofilm formation with optimized solutions.
Fig 5a. Optimized solutions (OS 1, 20% ethanol, 0.015625 μg/mL micafungin, 800 μg/mL doxycycline, and OS 2, 10% ethanol with 0.0313 μg/mL micafungin and 800 μg/mL doxycycline) were highly effective in preventing the formation of echinocandin-resistant clinical isolate and reference strain biofilms. Optimized solutions were incubated with C. albicans isolates for 24 hours at 37°C to prevent biofilm development and untreated positive controls were compared to drug treated groups with the same strain. Metabolic activity was measured with XTT reduction. * indicates p value of < 0.05. **0.0313 μg/mL was not significant compared to the control for strain 53264 only. Fig 5b. Optimized solutions (OS 1, 20% ethanol, 0.015625 μg/mL micafungin, 800 μg/mL doxycycline, and OS 2, 10% ethanol with 0.0313 μg/mL micafungin and 800 μg/mL doxycycline) significantly reduce the metabolic activity of echinocandin-resistant clinical isolates and reference strains. Optimized solutions were incubated with mature C. albicans biofilms for 24 hours at 37°C. Metabolic activity was measured with XTT reduction and untreated positive controls were compared to drug treated groups with the same strain. * indicates p value of < 0.05