Molecular Characterization and Transcriptional Regulation Analysis of the Bovine PDHB Gene
Fig 8
ChIP assay of MYOG and C/EBPß binding to PDHB promoter in vivo.
We analyzed immunoprecipitated products for MYOG (a) and C/EBPß (b) antibodies via RT-PCR. We analyzed immunoprecipitated products for MYOG (c) and C/EBPß (d) antibodies via ChIP-QPCR. We used total chromatin from muscle (a and c) and fat (b and d) as the input. We used normal mouse IgG as the negative control antibodies. **, P<0.01. Error bars represent the SD (n = 3).