Repeated Vaccination of Cows with HIV Env gp140 during Subsequent Pregnancies Elicits and Sustains an Enduring Strong Env-Binding and Neutralising Antibody Response
Fig 3
Inhibition of BrNAbs VRC01 and b12 binding to CD4bs epitopes by colostrum IgG from cow 7004 and 7008.
A) 7004 competition ELISA against VRC01, B) 7004 competition ELISA against b12, C) 7008 competition ELISA against VRC01, D) 7008 competition ELISA against b12. Competition ELISAs were performed by titrating the human neutralising mAb VRC01 and b12 against a constant background of 100 μg 7004 or 7008 purified colostrum IgG. The binding of VRC01 and b12 mAbs was detected using anti-human HRP conjugated antibody. The inhibition activity was determined by comparison of the amount of mAb VRC01 or b12 that showed the identical optical density to that of half of the maximum binding of 100 ng VRC01 or b12. Batch 0 is the pre-immune collected colostrum sample. Results represent the mean of 3 repeats and error bars show SD values. P values were calculated using one-way Anova followed by a Tukey HSD test post-test (****P < 0.0001, ***P < 0.001; **P ≤ 0.01; *P ≤ 0.05).