RPL13A and EEF1A1 Are Suitable Reference Genes for qPCR during Adipocyte Differentiation of Vascular Stromal Cells from Patients with Different BMI and HOMA-IR
Table 3
Descriptive analysis of Ct values variability from qPCR of undifferentiated and differentiated hVSCs from SAT of patients with different BMI and HOMA-IR index.
The table shows the statistics min, max, mean±SEM, standard variation (SD) and variance of the Ct values obtained after qPCR of undifferentiated and differentiated SAT-derived hVSCs in the four groups under investigation as well as the mean values for every reference gene assessed (right columns). Data show that RPL13A and EEF1A1 has the lowest variability (SD and variance values) suggesting more stability in their expression when compared to the other reference genes. VAT: visceral adipose tissue; SAT: subcutaneous adipose tissue. Lean L-IR: lean subjects with low-degree insulin resistance; Lean H-IR: lean subjects with high-degree insulin resistance; MO L-IR: morbid obese subjects with low-degree insulin resistance; MO H-IR: morbid obese with high-degree insulin resistance.