Redesigning Aldolase Stereoselectivity by Homologous Grafting
Fig 5
Model aldol reaction for testing the stereoselectivity of KDPG, KDPGal, and specific mutants.
The aldol reaction of propanal (1) and pyruvic acid (2) leads to the enantioselective generation of 3 and, by acidic lactonization, to butyrolactone 4.