A Plasmodium Promiscuous T Cell Epitope Delivered within the Ad5 Hexon Protein Enhances the Protective Efficacy of a Protein Based Malaria Vaccine
Fig 6
Antibody Responses induced by the vaccination regimen based on the Ad5HVRT53PyCMP vector.
CB6F1/J (n = 5 per group) mice were primed with 107 v.p. of Ad5 expressing the PyT53 epitope within the HVR2 hexon region and expressing PyCMP as a transgene or Ad5 expressing PyCMP. Both regimens included boosting immunizations at day 60 and at day 90 with the recombinant PyCMP protein. Top. Total IgG antibody titers 20 days after the final immunization expressed as log against the recombinant protein (A), MSP1 (B) and CSP (C) are presented. The differences between the groups were analyzed by unpaired Student’s t test, no significant statistical differences between the groups were found. Bottom. (D) Affinity index of the mice sera samples after treatment with 1M NH4SCN. (E) IgG1 and IgG2a titers were measured separately by isotype-specific ELISA, with sera obtained 20 days after the final immunization. Data are plotted as the ratio of the endpoint log titer of IgG2a/IgG1. Differences between the groups were analyzed by Student’s t test, significant differences are denoted by *(p<0.05).