DNA Topology and the Initiation of Virus DNA Packaging
Fig 3
A static bend at I2: permutation analysis of a 150 bp DNA fragment containing λ I1+, I2+, I2re30-35, or I2re18-50.
A. Diagram of the pBend plasmid showing the XbaI and SalI sites used to insert the I2 segments. Flanking the I2 inserts are repeated segments with the restriction enzyme target sites used to generate DNAs with permutations of I2 position. B. Relative mobilities of the permuted DNA fragments versus positions of the I2 segment. Relative mobility 1.0 indicates the mobility of a 150 bp DNA marker. The calculated bend angles [40] are given adjacent to the relevant mobility curve.