Loss of Panx1 Impairs Mammary Gland Development at Lactation: Implications for Breast Tumorigenesis
Fig 5
Panx1-/- mice have reduced cell proliferation during early lactation.
(A) The average number of alveolar lumens was significantly increased in early lactation compared to parturition in Panx1+/+ mice which was not observed in Panx1-/- mice. (B) Immunofluorescent analysis of the proliferation marker Ki67 (Red; parturition, green; lactation) revealed significantly reduced proliferation in mammary glands of Panx1-/- mice compared to controls during early lactation but not at parturition. (C) Immunofluorescent analysis of the apoptotic marker cleaved caspase3 revealed little apoptosis in the glands of knockout mice, similar to controls. Inserts represent positive controls in the involuting mammary gland. Hoescht (blue) denotes nuclei. N = 6. Scale bars = 50 um.