Loss of Panx1 Impairs Mammary Gland Development at Lactation: Implications for Breast Tumorigenesis
Fig 1
Panx1 is dynamically regulated in the mammary gland and ablated in Panx1-/- mice.
(A) Western blot analysis revealed that Panx1 is upregulated following the onset of pregnancy and remains elevated throughout lactation. Multiple bands on the western blot represent various Panx1 species due to changes in glycosylation (Gly0, Gly1 and Gly2). (B) Punctate staining observed for Panx1 (red) is not observed using pre-immune serum or following adsorption of the antibody with cognate peptide and does not significantly colocalize with the myoepithelial marker keratin14 (K14, green). Hoescht (blue) denotes nuclei. Scale bar = 50 um. (C, D) Western blot and real-time PCR analysis revealed that Panx1 is not expressed in Panx1-/- compared to Panx1+/+ mice.—represents HEK 293T cells and + represents these cells overexpressing Panx1. Values are means ± S.E.M. For all experiments, N = 3.